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126D

CRYSTAL STRUCTURE OF CATGGCCATG AND ITS IMPLICATIONS FOR A-TRACT BENDING MODELS

126D の概要
エントリーDOI10.2210/pdb126d/pdb
分子名称DNA (5'-D(*CP*AP*TP*GP*GP*CP*CP*AP*TP*G)-3') (2 entities in total)
機能のキーワードb-dna, double helix, dna
タンパク質・核酸の鎖数2
化学式量合計6090.01
構造登録者
Goodsell, D.S.,Kopka, M.L.,Cascio, D.,Dickerson, R.E. (登録日: 1993-06-14, 公開日: 1993-10-15, 最終更新日: 2024-02-07)
主引用文献Goodsell, D.S.,Kopka, M.L.,Cascio, D.,Dickerson, R.E.
Crystal structure of CATGGCCATG and its implications for A-tract bending models.
Proc.Natl.Acad.Sci.USA, 90:2930-2934, 1993
Cited by
PubMed Abstract: The single-crystal x-ray analysis of orthorhombic CATGGCCATG has revealed a previously unrecognized mode of intrinsic bending in DNA. The decamer shows a smooth bend of 23 degrees over the central four base pairs, caused by preferential stacking interactions at guanine bases. The bend is produced by a roll of base pairs along their long axes, in a direction that compresses the wide major groove of the double helix. This major-groove-compressing bend at GGC, plus the abundant crystallographic evidence that runs of successive adenine bases (A-tracts) are straight and unbent, requires rethinking of the models most commonly invoked to explain A-tract bending. A decade of excellent experimental work involving gel migration experiments, cyclization kinetics, and nucleosome phasing has clearly established that introduction of short A-tracts into a general DNA sequence in synchrony with the natural repeat of the helix leads to bending. But it does not logically and inevitably follow that the actual bending is to be found within these introduced A-tracts or even at junctions with general-sequence B-DNA.
PubMed: 8464909
DOI: 10.1073/pnas.90.7.2930
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2 Å)
構造検証レポート
Validation report summary of 126d
検証レポート(詳細版)ダウンロードをダウンロード

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件を2024-12-25に公開中

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