11AA

Yeast co-transcriptional pre-60S assembly intermediate Nop12 RNP

11AA の概要

• エントリーDOI: 10.2210/pdb11aa/pdb
• EMDBエントリー: 75580
• 分子名称: 25S ribosomal RNA, Ribosome biogenesis protein BRX1, 60S ribosomal protein L36-A, ... (18 entities in total)
• 機能のキーワード: ribosome biogenesis, co-transcriptional assembly, rna-binding protein, nucleolar rnp, ribosome
• 由来する生物種: Saccharomyces cerevisiae S288C; 詳細
• タンパク質・核酸の鎖数: 17
• 化学式量合計: 1795108.14

構造登録者

Piwowarczyk, R.,Klinge, S. (登録日: 2026-02-13, 公開日: 2026-07-01)

主引用文献

Piwowarczyk, R.,Klinge, S.
Mechanism for the initiation of co-transcriptional pre-60 S assembly.
Biorxiv, 2026

PubMed Abstract: Eukaryotic ribosomal large subunit (60 ) assembly requires an internal transcribed spacer 2 (ITS2) to license both nucleolar and nuclear pre-60 assembly intermediates. The underlying molecular mechanisms responsible for nucleation of pre-60 assembly, quality control, and installation of ITS2 during co-transcriptional stages remain unknown. Here we report the earliest co-transcriptional assembly intermediates of the eukaryotic 60 subunits. Together with biochemical assays, our data reveal the architecture of co-transcriptional pre-60 assembly initiation and progression, as well as the molecular logic of an assembly checkpoint. This study highlights an evolutionary solution by which complex RNA folding processes can be parallelized and integrated via biological AND-gating.

PubMed: 42239465
DOI: 10.64898/2026.05.22.727207

実験手法

ELECTRON MICROSCOPY (2.78 Å)