4NC3
Crystal structure of the 5-HT2B receptor solved using serial femtosecond crystallography in lipidic cubic phase.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Chimera protein of human 5-hydroxytryptamine receptor 2B and E. Coli soluble cytochrome b562 | polymer | 430 | 48283.3 | 1 | UniProt (P41595) UniProt (P0ABE7) Pfam (PF00001) Pfam (PF07361) In PDB | Homo sapiens (Human) | |
| 2 | A | PALMITIC ACID | non-polymer | 256.4 | 1 | Chemie (PLM) | |||
| 3 | A | Ergotamine | non-polymer | 581.7 | 1 | Chemie (ERM) | |||
| 4 | A | CHOLESTEROL | non-polymer | 386.7 | 1 | Chemie (CLR) | |||
| 5 | A | (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate | non-polymer | 356.5 | 4 | Chemie (OLC) | |||
| 6 | A | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
| 7 | A | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 1 | Chemie (TRS) | |||
| 8 | A | OLEIC ACID | non-polymer | 282.5 | 3 | Chemie (OLA) | |||
| 9 | A | DIACYL GLYCEROL | non-polymer | 625.0 | 2 | Chemie (DGA) | |||
| 10 | water | water | 18.0 | 7 | Chemie (HOH) |
Sequence modifications
A: 36 - 248 (UniProt: P41595)
A: 1001 - 1106 (UniProt: P0ABE7)
A: 314 - 405 (UniProt: P41595)
| PDB | External Database | Details |
|---|---|---|
| Asp 26 | - | expression tag |
| Tyr 27 | - | expression tag |
| Lys 28 | - | expression tag |
| Asp 29 | - | expression tag |
| Asp 30 | - | expression tag |
| Asp 31 | - | expression tag |
| Asp 32 | - | expression tag |
| Gly 33 | - | expression tag |
| Ala 34 | - | expression tag |
| Pro 35 | - | expression tag |
| Trp 144 | Met 144 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Trp 1007 | Met 29 | engineered mutation |
| Ile 1102 | His 124 | engineered mutation |
| Leu 1106 | Arg 128 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 406 | - | expression tag |
| Arg 407 | - | expression tag |
| Pro 408 | - | expression tag |
| Leu 409 | - | expression tag |
| Glu 410 | - | expression tag |
| Val 411 | - | expression tag |
| Leu 412 | - | expression tag |
| Phe 413 | - | expression tag |
| Gln 414 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 48283.3 | |
| Non-Polymers* | Number of molecules | 14 |
| Total formula weight | 4976.6 | |
| All* | Total formula weight | 53259.9 |
