2Y8Q

Structure of the regulatory fragment of mammalian AMPK in complex with one ADP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	5'-AMP-ACTIVATED PROTEIN KINASE CATALYTIC SUBUNIT ALPHA-1	polymer	173	19486.8	1	UniProt (P54645) Pfam (PF16579) In PDB	RATTUS NORVEGICUS (NORWAY RAT)	AMPK SUBUNIT ALPHA-1
2	B	5'-AMP-ACTIVATED PROTEIN KINASE SUBUNIT BETA-2	polymer	87	10040.8	1	UniProt (O43741) Pfam (PF04739) In PDB	HOMO SAPIENS (HUMAN)	AMPK SUBUNIT BETA-2
3	E	5'-AMP-ACTIVATED PROTEIN KINASE SUBUNIT GAMMA-1	polymer	330	37434.1	1	UniProt (P80385) Pfam (PF00571) In PDB	RATTUS NORVEGICUS (NORWAY RAT)	AMPK GAMMA1, AMPK SUBUNIT GAMMA-1, AMPKG
4	E	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
5	E	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	1	Chemie (AMP)
6		water	water		18.0	56	Chemie (HOH)

Sequence modifications

A: 396 - 544 (UniProt: P54645)

PDB	External Database	Details
Met 378	-	expression tag
Ser 379	-	expression tag
His 380	-	expression tag
His 381	-	expression tag
His 382	-	expression tag
His 383	-	expression tag
His 384	-	expression tag
His 385	-	expression tag
Ser 386	-	expression tag
Gly 387	-	expression tag
Leu 388	-	expression tag
Val 389	-	expression tag
Pro 390	-	expression tag
Arg 391	-	expression tag
Gly 392	-	expression tag
Ser 393	-	expression tag
Met 394	-	expression tag
Ala 395	-	expression tag
Asn 545	-	expression tag
Ser 546	-	expression tag
Cys 547	-	expression tag
Thr 548	-	expression tag
Val 549	-	expression tag
Asn 550	-	expression tag

B: 187 - 270 (UniProt: O43741)

PDB	External Database	Details
Met 186	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	66961.7
Non-Polymers*	Number of molecules	2
	Total formula weight	774.4
All*	Total formula weight	67736.2

*Water molecules are not included.