5GWI

Structure of a Human topoisomerase IIbeta fragment in complex with DNA and E7873R

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003918	molecular_function	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
A	0005524	molecular_function	ATP binding
A	0006259	biological_process	DNA metabolic process
A	0006265	biological_process	DNA topological change
B	0003677	molecular_function	DNA binding
B	0003918	molecular_function	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
B	0005524	molecular_function	ATP binding
B	0006259	biological_process	DNA metabolic process
B	0006265	biological_process	DNA topological change

Functional Information from PDB Data

site_id	AC1
Number of Residues	12
Details	binding site for residue N2R A 1301

Chain	Residue
A	GLY478
D	DG10
F	DA12
F	DG13
A	ASP479
A	LEU502
A	ARG503
A	GLN778
A	MET782
A	N2N1302
C	DC8
D	DT9

---

site_id	AC2
Number of Residues	12
Details	binding site for residue N2N A 1302

Chain	Residue
A	GLY478
A	ASP479
A	LEU502
A	ARG503
A	GLN778
A	MET782
A	N2R1301
C	DC8
D	DT9
D	DG10
F	DA12
F	DG13

---

site_id	AC3
Number of Residues	3
Details	binding site for residue MG A 1303

Chain	Residue
A	ASP557
A	ASP559
A	HOH1412

---

site_id	AC4
Number of Residues	2
Details	binding site for residue MG A 1304

Chain	Residue
A	ASN867
A	GLY868

---

site_id	AC5
Number of Residues	11
Details	binding site for residue N2R B 1301

Chain	Residue
B	GLY478
B	ASP479
B	ARG503
B	GLN778
B	MET782
B	N2N1302
D	DA12
D	DG13
E	DC8
F	DT9
F	DG10

---

site_id	AC6
Number of Residues	4
Details	binding site for residue MG B 1303

Chain	Residue
B	ASP557
B	ASP559
B	HOH1420
E	HOH201

---

site_id	AC7
Number of Residues	12
Details	binding site for residue N2N B 1302

Chain	Residue
B	GLY478
B	ASP479
B	ARG503
B	GLN778
B	MET781
B	MET782
B	N2R1301
D	DA12
D	DG13
E	DC8
F	DT9
F	DG10

---

site_id	AC8
Number of Residues	2
Details	binding site for residue MG D 101

Chain	Residue
D	HOH201
D	HOH203

---

site_id	AC9
Number of Residues	1
Details	binding site for residue MG E 101

Chain	Residue
E	DG5

---

Functional Information from PROSITE/UniProt

site_id	PS00177
Number of Residues	9
Details	TOPOISOMERASE_II DNA topoisomerase II signature. LTEGDSAKS

Chain	Residue	Details
A	LEU475-SER483

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: O-(5'-phospho-DNA)-tyrosine intermediate => ECO:0000255|PROSITE-ProRule:PRU01384, ECO:0000269|PubMed:21778401

Chain	Residue	Details
A	TYR821
B	TYR821

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00995

Chain	Residue	Details
A	GLU477
B	GLU477

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00995, ECO:0000269|PubMed:21778401

Chain	Residue	Details
A	ASP557
A	ASP559
B	ASP557
B	ASP559

---

site_id	SWS_FT_FI4
Number of Residues	12
Details	SITE: Interaction with DNA

Chain	Residue	Details
A	LYS505
B	LYS678
B	LYS739
B	TRP947
A	ASN508
A	ARG677
A	LYS678
A	LYS739
A	TRP947
B	LYS505
B	ASN508
B	ARG677

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	SITE: Interaction with DNA => ECO:0000255|PROSITE-ProRule:PRU00995

Chain	Residue	Details
A	TYR773
B	TYR773

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	SITE: Transition state stabilizer

Chain	Residue	Details
A	ARG820
B	ARG820

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	SITE: Important for DNA bending; intercalates between base pairs of target DNA

Chain	Residue	Details
A	ILE872
B	ILE872

---

site_id	SWS_FT_FI8
Number of Residues	14
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733

Chain	Residue	Details
A	LYS595
B	LYS630
B	LYS641
B	LYS671
B	LYS707
B	LYS1087
A	LYS630
A	LYS641
A	LYS671
A	LYS707
A	LYS1087
B	LYS595

---

site_id	SWS_FT_FI9
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:25772364, ECO:0007744|PubMed:28112733

Chain	Residue	Details
A	LYS600
A	LYS638
B	LYS600
B	LYS638

---