9ZUH

Measles Virus Fusion Glycoprotein Postfusion Core (L454W Variant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-17
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.979338
Spacegroup name	P 1 21 1
Unit cell lengths	57.688, 52.091, 72.711
Unit cell angles	90.00, 91.59, 90.00

Refinement procedure

Resolution	57.670 - 2.130
R-factor	0.2188
Rwork	0.215
R-free	0.26350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.058
RMSD bond angle	0.642
Data reduction software	XDS (Jan 10, 2022 (BUILT 20220820))
Data scaling software	Aimless (0.7.7)
Phasing software	PHASER (1.21.2_5419)
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.670	2.167
High resolution limit [Å]	2.130	2.130
Rmerge	0.211	0.788
Rmeas	0.298	1.111
Rpim	0.210	0.783
Number of reflections	23573	1152
<I/σ(I)>	2.4	1.2
Completeness [%]	96.6	96.8
Redundancy	2.8	2.9
CC(1/2)	0.967	0.410

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	281	30M Magnesium chloride hexahydrate; 30M Calcium chloride dihydrate, 100mM Imidazole; 100mM MES monohydrate (acid), 12.5% v/v MPD; 12.5% PEG 1000; 12.5% w/v PEG 3350 (pH 6.5)