9ZSN
Flavophos biosynthetic protein BsfD with CoA and OBPn (48 h)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-05 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 86.118, 110.462, 72.659 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.740 |
| R-factor | 0.18935 |
| Rwork | 0.189 |
| R-free | 0.20392 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.064 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 1.767 |
| High resolution limit [Å] | 1.737 | 1.737 |
| Rmerge | 0.068 | 1.212 |
| Rmeas | 0.072 | 1.267 |
| Number of reflections | 72100 | 3538 |
| <I/σ(I)> | 27.6 | |
| Completeness [%] | 100.0 | |
| Redundancy | 14.5 | |
| CC(1/2) | 0.999 | 0.829 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 287 | 25% PEG 4000 100 mM calcium acetate 100 mM sodium acetate, pH=4.5 |
