9ZSH
Flavophos biosynthetic protein BsfD with CoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-15 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 53.436, 119.602, 121.565 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.500 |
| R-factor | 0.19049 |
| Rwork | 0.190 |
| R-free | 0.19865 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.043 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.900 | 1.504 |
| High resolution limit [Å] | 1.499 | 1.499 |
| Rmerge | 0.096 | 0.577 |
| Rmeas | 0.103 | 0.680 |
| Number of reflections | 62636 | 1798 |
| <I/σ(I)> | 13.4 | 2 |
| Completeness [%] | 99.8 | |
| Redundancy | 9.5 | |
| CC(1/2) | 0.997 | 0.599 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 287 | 1.6 M lithium sulfate 100 mM Tris-HCl, pH=8.5 |
