9ZRP

Neutralizing W049 antibody Fab fragment in complex with West Nile Virus EDIII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-02-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	97.179, 112.691, 129.888
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.550 - 2.500
R-factor	0.2172
Rwork	0.215
R-free	0.26390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.630
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.550	2.580
High resolution limit [Å]	2.500	2.500
Number of reflections	49049	4426
<I/σ(I)>	8.7
Completeness [%]	98.2
Redundancy	6.9
CC(1/2)	0.996	0.487

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	12% w/v PEG3,350 and 0.1M disodium DL-malate