9ZRM
Potently Neutralizing W010 antibody Fab fragment in complex with West Nile Virus EDIII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-12-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.879, 56.862, 67.871 |
| Unit cell angles | 66.46, 89.39, 70.24 |
Refinement procedure
| Resolution | 37.140 - 1.800 |
| R-factor | 0.1757 |
| Rwork | 0.174 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.670 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419+SVN) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.140 | 1.800 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 42885 | 2502 |
| <I/σ(I)> | 11.9 | |
| Completeness [%] | 90.8 | |
| Redundancy | 3.9 | |
| CC(1/2) | 0.997 | 0.943 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 20% w/v PEG4,000, 0.2M disodium malonate |
