9ZPE

KRAS G12V Mutant in Complex with GDP and Compound 8.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-05-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.195
Spacegroup name	P 1 21 1
Unit cell lengths	52.723, 53.518, 62.175
Unit cell angles	90.00, 90.06, 90.00

Refinement procedure

Resolution	52.720 - 1.910
R-factor	0.1962
Rwork	0.194
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.845
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.175	2.194
High resolution limit [Å]	1.909	1.909
Rmerge	0.110	0.777
Rmeas	0.126	0.886
Rpim	0.061	0.420
Number of reflections	13816	691
<I/σ(I)>	7	1.6
Completeness [%]	88.0
Redundancy	4.2
CC(1/2)	0.993	0.595

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	294	30% w/v PEG4K, 0.2 M (NH4)2SO4, 0.1 M Na Acetate pH 4.6