9ZN0

Crystal structure of the Commd10 S50E HN domain mutant in domain swapped conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-12-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95373
Spacegroup name	P 1 21 1
Unit cell lengths	43.759, 92.173, 59.815
Unit cell angles	90.00, 111.34, 90.00

Refinement procedure

Resolution	40.760 - 2.120
R-factor	0.1943
Rwork	0.190
R-free	0.24330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.858
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.700	2.180
High resolution limit [Å]	2.120	2.120
Rpim	0.026	0.152
Number of reflections	24828	1949
<I/σ(I)>	12.7	3.7
Completeness [%]	98.6
Redundancy	3.8
CC(1/2)	0.999	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	293	0.1 M Tris (pH 8.5), 0.1 M Mg formate, and 30% PE14/4, with 10% glycerol used as cryoprotectant