9ZMZ

Crystal structure of the human Commd4 HN domain in a domain swapped conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-12-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95373
Spacegroup name	P 32 2 1
Unit cell lengths	51.535, 51.535, 79.309
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.630 - 2.120
R-factor	0.2371
Rwork	0.230
R-free	0.30430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.030
RMSD bond angle	2.522
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.630	2.180
High resolution limit [Å]	2.120	2.120
Rpim	0.021	0.361
Number of reflections	7291	562
<I/σ(I)>	16.4	1.6
Completeness [%]	99.6
Redundancy	19.1
CC(1/2)	0.999	0.494

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	293	0.1 M Tris (pH 8.0) and 28% PEG4000