9ZMD

Equine Serum Albumin with Copper(II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-16
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.28291
Spacegroup name	P 61
Unit cell lengths	93.428, 93.428, 141.752
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.985 - 2.650
Rwork	0.191
R-free	0.25130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	1.067
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.000	2.400
High resolution limit [Å]	2.360	2.360
Rmerge	0.081
Rpim	0.037
Number of reflections	28717	1291
<I/σ(I)>	17.7
Completeness [%]	98.8
Redundancy	5.5
CC(1/2)		0.480

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	291.15	0.2 M lithium sulfate, 0.1 M Tris-HCl, pH 7.4, 2.2 M ammonium sulfate, 5 mM copper(II) chloride mixed 1:1 with 36 mg/mL protein, 10 mM Tris-HCl, 150 mM sodium chloride