9ZMC

Crystal structure of Fab 7118 in complex with major repeat region (NANP6) from circumsporozoite protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-03-20
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	74.150, 83.395, 89.549
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.700 - 2.000
R-factor	0.1822
Rwork	0.180
R-free	0.21740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.275
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	1.990	1.990
Number of reflections	38496	1871
<I/σ(I)>	38
Completeness [%]	99.0
Redundancy	6
CC(1/2)	0.990	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1 M sodium cacodylate (pH 6.5), 0.16 M calcium acetate, 14.4% (w/v) PEG 8000, and 20% (v/v) glycerol