9ZM9
Crystal structure of Fab 7160 in complex with short major repeat region (NPNA3) from circumsporozoite protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-01 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.92 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 188.010, 52.348, 102.986 |
| Unit cell angles | 90.00, 110.07, 90.00 |
Refinement procedure
| Resolution | 26.790 - 1.880 |
| R-factor | 0.2009 |
| Rwork | 0.200 |
| R-free | 0.24520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.903 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.880 | 1.900 |
| Number of reflections | 75043 | 3573 |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 99.7 | |
| Redundancy | 6.2 | |
| CC(1/2) | 0.980 | 0.690 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2 M sodium acetate (pH 7.0) and 20% (w/v) PEG 3350. |
