9ZM7

Crystal structure of Fab 7160 in complex with junctional region from circumsporozoite protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 21 2 21
Unit cell lengths	88.190, 105.127, 405.183
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.880 - 2.300
R-factor	0.2291
Rwork	0.229
R-free	0.27480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.526
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.340
High resolution limit [Å]	2.300	2.300
Number of reflections	168500	8359
<I/σ(I)>	15.1
Completeness [%]	99.9
Redundancy	12
CC(1/2)	0.980	0.730

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	0.2 M sodium dihydrogen phosphate (pH 4.5) and 20% (w/v) polyethylene glycol (PEG) 3350