9ZM5

Crystal structure of the Arabidopsis NPR1-NIMIN1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 2.0.1
Synchrotron site	ALS
Beamline	2.0.1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2023-12-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	118.483, 144.926, 145.329
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	3.500 - 3.330
Rwork	0.228
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.860	45.860
High resolution limit [Å]	3.330	3.330
Number of reflections	16411	16411
<I/σ(I)>	0.285
Completeness [%]	97.9
Redundancy	2.3
CC(1/2)	0.833	0.833

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277.15	0.2M Ammonium Chloride, 0.1M HEPES pH 7.5, 25% w/v PEG 3350