9ZM0

Crystal structure of monomeric Atg23

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-01-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97988
Spacegroup name	P 1 21 1
Unit cell lengths	50.409, 30.101, 91.244
Unit cell angles	90.00, 97.15, 90.00

Refinement procedure

Resolution	46.290 - 2.100
R-factor	0.2412
Rwork	0.238
R-free	0.29760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.531
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.290	2.170
High resolution limit [Å]	2.100	2.100
Rmerge	0.115	0.970
Rpim	0.103	0.831
Number of reflections	16342	1324
<I/σ(I)>	5.6
Completeness [%]	100.0	100
Redundancy	3.8	3.9
CC(1/2)	0.990	0.650

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.02 M xylitol, D-fructose, D-sorbitol, myo-inositol, L-rhamnose each; 9% PEG8000, 18% 1,5-pentanediol, 0.07 M MOPSO, 0.03 M Bis-Tris