9ZLL

Complex of N-terminal BrxC walker B, BrxB, and N-termianl PglZ from the Acinetobacter BREX system

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-10-15
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.97741
Spacegroup name	I 1 2 1
Unit cell lengths	149.248, 114.986, 194.050
Unit cell angles	90.00, 108.03, 90.00

Refinement procedure

Resolution	49.680 - 2.740
R-factor	0.2068
Rwork	0.206
R-free	0.24760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.679
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.21.1_5286+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.093	0.780
Rmeas	0.101	0.840
Rpim	0.038	0.320
Number of reflections	77820	5362
<I/σ(I)>	14.3	2.2
Completeness [%]	99.9	99.7
Redundancy	6.8	6.7
CC(1/2)	0.998	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	250 mM NaCl, 100 mM HEPES pH 8.5, 21% PEG3350