9ZLI

Crystal structure of DCAF1 in complex with SDIPTAC C11

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-002+
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-07-28
Detector	DECTRIS EIGER R 1M
Wavelength(s)	1.54184
Spacegroup name	C 1 2 1
Unit cell lengths	83.921, 145.150, 222.130
Unit cell angles	90.00, 90.15, 90.00

Refinement procedure

Resolution	30.380 - 2.070
R-factor	0.2422
Rwork	0.242
R-free	0.27840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.883
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.380	2.180
High resolution limit [Å]	2.070	2.070
Rmerge	0.221	2.333
Rmeas	0.247	0.247
Rpim	0.108	0.108
Number of reflections	161325	23519
<I/σ(I)>	5.2
Completeness [%]	99.9
Redundancy	5.1
CC(1/2)	0.990	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	294.15	0.1M magnesium chloride hexahydrate, 0.1M potassium chloride, 0.1M PIPES, pH 7.0, 20% v/v PEG smear medium