9ZL4
Crystal structure of PRMT5:MEP50 in complex with MTA and TNG456
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 100.510, 138.260, 178.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.660 - 1.950 |
| R-factor | 0.1793 |
| Rwork | 0.176 |
| R-free | 0.23610 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.872 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.660 | 2.040 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.093 | 0.777 |
| Rpim | 0.026 | 0.305 |
| Number of reflections | 65491 | 3275 |
| <I/σ(I)> | 18.4 | 2.3 |
| Completeness [%] | 72.6 | 30.3 |
| Redundancy | 12.9 | |
| CC(1/2) | 0.999 | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 10% PEG 4000, 0.1 M sodium citrate (pH 6.), 0.2 M magnesium chloride |
