9ZL3
Crystal structure of PRMT5:MEP50 in complex with MTA and oxamide compound 18
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 102.367, 138.128, 178.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.620 - 1.710 |
| R-factor | 0.1914 |
| Rwork | 0.189 |
| R-free | 0.23900 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.720 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 109.000 | 1.951 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmerge | 0.070 | 1.202 |
| Rpim | 0.026 | 0.467 |
| Number of reflections | 67184 | 3360 |
| <I/σ(I)> | 17.9 | 1.9 |
| Completeness [%] | 49.4 | 7.6 |
| Redundancy | 8.3 | 7.4 |
| CC(1/2) | 0.999 | 0.669 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 293 | 10% PEG 4000, 0.1 M sodium citrate (pH 6.0), 0.2 M magnesium chloride |
