9ZL2
Crystal structure of PRMT5:MEP50 in complex with MTA and oxamide compound 8
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-08 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97959 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 101.420, 136.780, 178.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.940 - 3.180 |
| R-factor | 0.2617 |
| Rwork | 0.260 |
| R-free | 0.29340 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.875 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.940 | 3.400 |
| High resolution limit [Å] | 3.180 | 3.180 |
| Rmerge | 0.101 | 0.690 |
| Rmeas | 0.110 | 0.746 |
| Rpim | 0.042 | 0.282 |
| Number of reflections | 21184 | 3779 |
| <I/σ(I)> | 12.2 | 2.4 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 6.7 | 6.9 |
| CC(1/2) | 0.999 | 0.970 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 293 | 10% PEG 4000, 0.1 M sodium citrate (pH 6.0), 0.2 M magnesium chloride |
