9ZKI

Human NRAS specific T cell receptor N17.2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.92019
Spacegroup name	P 64 2 2
Unit cell lengths	286.402, 286.402, 103.909
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.800 - 2.910
R-factor	0.2504
Rwork	0.249
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.073
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.800	2.970
High resolution limit [Å]	2.910	2.910
Number of reflections	51289	1330
<I/σ(I)>	9.8
Completeness [%]	99.9
Redundancy	20
CC(1/2)	0.995	0.474

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	20 % PEG 4000, 50 mM lithium sulfate, 100 mM hepes 7