9ZG5

Structure of superfolder GFP bound to nanobody 15

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU R-AXIS IV
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2025-05-14
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 32
Unit cell lengths	169.050, 169.050, 70.790
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.400 - 3.400
R-factor	0.2968
Rwork	0.295
R-free	0.33130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.444
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.400	34.000	3.490
High resolution limit [Å]	3.400	15.210	3.400
Rmerge		0.083	0.519
Rmeas	0.361	0.098	0.602
Number of reflections	31040	313	2287
<I/σ(I)>	4.4
Completeness [%]	99.8
Redundancy	3.8
CC(1/2)	0.943	0.992	0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2 M ammonium sulfate , 0.1 M HEPES pH 7.5, 29 % PEG 3350