9ZEM
HeliScrew4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-10-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.339, 62.620, 45.980 |
| Unit cell angles | 90.00, 96.42, 90.00 |
Refinement procedure
| Resolution | 45.690 - 2.200 |
| R-factor | 0.2009 |
| Rwork | 0.197 |
| R-free | 0.23690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.076 |
| Data reduction software | RAPD |
| Data scaling software | RAPD |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.690 | 2.090 |
| High resolution limit [Å] | 2.046 | 2.050 |
| Rmeas | 0.344 | |
| Rpim | 0.143 | 0.509 |
| Number of reflections | 68979 | 765 |
| <I/σ(I)> | 5.29 | 3.17 |
| Completeness [%] | 92.4 | 91.24 |
| Redundancy | 5.4 | 5.6 |
| CC(1/2) | 0.950 | 0.392 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.8 M ammonium citrate tribasic, pH 7.0 |
