9ZDM

RhsF/RhsFi complex from Chromobacterium violaceum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-14
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	P 1
Unit cell lengths	34.831, 52.923, 66.390
Unit cell angles	72.03, 86.91, 71.84

Refinement procedure

Resolution	47.850 - 1.850
R-factor	0.1917
Rwork	0.190
R-free	0.21940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.013
Data reduction software	XDS (5.15.9)
Data scaling software	XDS (5.15.9)
Phasing software	PHENIX (1.21_5207)
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.850	1.970
High resolution limit [Å]	1.850	1.920
Rmerge	0.087
Rmeas	0.104
Rpim	0.056
Number of reflections	34223	1831
<I/σ(I)>	6.8
Completeness [%]	94.6
Redundancy	3.4
CC(1/2)		0.769

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	8.33 mg/mL protein complex; 83.3 mM NaCl; 67 mM MMT (malic acid, 2-(N-morpholino)ethanesulfonic acid); 16.7 mM Tris-Cl; 6.7% ethanol; 16.7% PEG1500; pH 6.0