9ZDG
Calcium biosensor with a fluorescence-lifetime readout
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2023-01-09 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 79.330, 79.330, 170.140 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.350 - 2.330 |
| R-factor | 0.2149 |
| Rwork | 0.212 |
| R-free | 0.25220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.431 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1-4487-000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.350 | 2.413 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Number of reflections | 26816 | 2408 |
| <I/σ(I)> | 26.66 | |
| Completeness [%] | 98.3 | |
| Redundancy | 2 | |
| CC(1/2) | 0.999 | 0.793 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 3.5 M potassium acetate, pH 7.5 |
