9ZCT

Crystal structure of HOPS subunits Vps33 and Vps16 in complex with the Nyv1 SNARE motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-01
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97242
Spacegroup name	P 41 21 2
Unit cell lengths	99.492, 99.492, 308.937
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.500 - 2.900
R-factor	0.192
Rwork	0.189
R-free	0.25020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.907
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.500	3.040
High resolution limit [Å]	2.900	2.900
Rmerge	0.236	1.921
Rmeas	0.248	2.015
Rpim	0.075	0.600
Total number of observations	387603	51139
Number of reflections	35477	4627
<I/σ(I)>	8.9	1.5
Completeness [%]	99.9
Redundancy	10.9	11.1
CC(1/2)	0.995	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1 M HEPES, pH 7.5, 0.5 M LiCl, 13% PEG8000