9ZCM

Human NRAS specific T cell receptor N17.3.2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-30
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9202
Spacegroup name	P 32 2 1
Unit cell lengths	109.445, 109.445, 96.747
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.780 - 1.800
R-factor	0.1959
Rwork	0.194
R-free	0.22640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.890
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.780	1.864
High resolution limit [Å]	1.800	1.800
Number of reflections	62273	6164
<I/σ(I)>	19.91
Completeness [%]	100.0
Redundancy	2
CC(1/2)	1.000	0.767

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	0.2 M sodium formate, 0.1 M bicine (pH 8.5), and 20% w/v PEG monomethyl ether 5000