9ZCK

WNK1/S382A in cesium formate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-06-30
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	2.156
Spacegroup name	P 1
Unit cell lengths	38.643, 57.833, 65.513
Unit cell angles	89.11, 89.34, 89.38

Refinement procedure

Resolution	33.450 - 2.200
R-factor	0.1791
Rwork	0.177
R-free	0.22590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.839
Data reduction software	xia2
Data scaling software	xia2
Phasing software	MOLREP
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.600	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.053	0.119
Number of reflections	28791	1294
<I/σ(I)>	2.4
Completeness [%]	97.3	90.2
Redundancy	4.7	3.7
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	289	50 mM Tris/HCl pH 7.4 5 mM EDTA 200 mM cesium formate 20% PEG 3350