9ZB1

Crystal structure of human MGAT1 in complex with UDP-GlcNAc.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.93925
Spacegroup name	P 2 21 21
Unit cell lengths	54.925, 113.052, 129.542
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.400 - 2.000
R-factor	0.172
Rwork	0.170
R-free	0.21970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.893
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	REFMAC
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.400	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.167	0.966
Rmeas	0.188	1.106
Number of reflections	55419	4057
<I/σ(I)>	8.8	2.3
Completeness [%]	100.0	100
Redundancy	8.6	7.9
CC(1/2)	0.994	0.758

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	16% PEG 4000, 100 mM Tris-HCl (pH 8.5), and 200 mM sodium acetate