9ZAP
cI(NTD) of Phage VP882
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-31 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.920 |
| Spacegroup name | P 32 |
| Unit cell lengths | 44.020, 44.020, 65.798 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.910 - 1.370 |
| R-factor | 0.1474 |
| Rwork | 0.145 |
| R-free | 0.18370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.041 |
| Data reduction software | FAST_DP |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.910 | 1.410 |
| High resolution limit [Å] | 1.370 | 1.370 |
| Rmerge | 0.070 | 0.621 |
| Rmeas | 0.075 | 0.673 |
| Number of reflections | 29691 | 2181 |
| <I/σ(I)> | 14.6 | 2.3 |
| Completeness [%] | 99.9 | 98.3 |
| Redundancy | 7.8 | 6.8 |
| CC(1/2) | 0.999 | 0.812 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M BIS-TRIS pH 5.5, 0.1 M Lithium sulfate, 20% PEG 3350 |
