9Z73
Structure of V. cholerae CapS (form 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 79.276, 98.192, 278.996 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.380 - 1.840 |
| R-factor | 0.1896 |
| Rwork | 0.188 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.518 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.680 | 1.876 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.092 | 2.358 |
| Rmeas | 0.099 | 2.764 |
| Number of reflections | 93914 | 4570 |
| <I/σ(I)> | 12.3 | 1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7 | 7.2 |
| CC(1/2) | 0.995 | 0.355 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Ammonium sulfate, 0.1 M sodium acetate pH 4.6, and 17% (w/v) PEG 4000 |
