9Z71
Structure of E. roggenkampii CapS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-29 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 55.826, 55.826, 184.515 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.330 - 1.550 |
| R-factor | 0.1678 |
| Rwork | 0.167 |
| R-free | 0.19020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.924 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.010 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.101 | 1.260 |
| Rmeas | 0.107 | 1.353 |
| Number of reflections | 25795 | 1209 |
| <I/σ(I)> | 17.6 | 1.6 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 17.1 | 7.6 |
| CC(1/2) | 0.999 | 0.660 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Ammonium sulfate and 13% (w/v) PEG 4000 |
