9Z6R

Crystal Structure of CBS domain containing protein from Burkholderia phymatum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 19-ID
Synchrotron site	NSLS-II
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-11-01
Detector	DECTRIS EIGER2 XE 9M
Wavelength(s)	0.9786
Spacegroup name	P 1 21 1
Unit cell lengths	41.947, 40.742, 67.579
Unit cell angles	90.00, 93.11, 90.00

Refinement procedure

Resolution	41.890 - 1.390
R-factor	0.1641
Rwork	0.162
R-free	0.19370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.058
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (2.0_5875)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.890	1.410
High resolution limit [Å]	1.390	1.390
Rmerge	0.032	0.970
Rmeas	0.035	1.062
Rpim	0.013	0.424
Total number of observations	310724	13692
Number of reflections	46058	2261
<I/σ(I)>	21.5	1.7
Completeness [%]	99.9
Redundancy	6.7	6.1
CC(1/2)	1.000	0.762

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	291	Berkeley A2: 100 mM sodium acetate, pH 4.5, 2 M sodium formate. BuphA.00062.a.B2.PW39454 at 37 mg/mL. plate 20515 A2 drop 2, Puck: PSL-0210, Cryo: 80% crystallant + 20% PEG 200