9Z6E

Crystal structure of apo LIMK1 mutant D460N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.18079
Spacegroup name	P 1 21 1
Unit cell lengths	54.376, 59.487, 103.030
Unit cell angles	90.00, 102.66, 90.00

Refinement procedure

Resolution	51.840 - 2.260
R-factor	0.1945
Rwork	0.192
R-free	0.24520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.425
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.840	2.460
High resolution limit [Å]	2.260	2.260
Number of reflections	20198	1015
<I/σ(I)>	12.6
Completeness [%]	66.7
Redundancy	3.9
CC(1/2)	0.990	0.560

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289.15	Morpheus condition A8 consisted of 0.06 M divalents, 0.1 M Buffer System 2, pH 7.5, and 37.5% (v/v) Precipitant Mix 4. Morpheus condition D12 consisted of 0.12 M alcohols, 0.1 M Buffer System 3, pH 8.5, and 37.5% Precipitant Mix 4.