9Z5W
Crystal structure of HRAS in complex with N-LHY affibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 19-ID |
| Synchrotron site | NSLS-II |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-06-03 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97860 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 55.529, 55.529, 280.680 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.090 - 1.800 |
| R-factor | 0.20382 |
| Rwork | 0.202 |
| R-free | 0.22385 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.840 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 280.640 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.106 | 3.437 |
| Rmeas | 0.107 | 3.539 |
| Rpim | 0.018 | 0.815 |
| Number of reflections | 25123 | 1177 |
| <I/σ(I)> | 20.7 | |
| Completeness [%] | 100.0 | 97.7 |
| Redundancy | 34.5 | 19.9 |
| CC(1/2) | 0.999 | 0.543 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 289 | 0.2 M AMMONIUM NITRATE, 20 % (W/V) PEG3350 |
