9Z2I
Crystal Structure of the Poly(Hexamethylene Adipamide) (Nylon66) Hydrolase Nyl50 in its Apo form at Room Temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2024-10-22 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 53.755, 96.398, 104.618 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.270 - 2.200 |
| R-factor | 0.15047 |
| Rwork | 0.148 |
| R-free | 0.19645 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.046 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.720 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.199 | 0.786 |
| Rmeas | 0.207 | 0.820 |
| Rpim | 0.056 | 0.226 |
| Total number of observations | 365287 | 30780 |
| Number of reflections | 27717 | 2347 |
| <I/σ(I)> | 11.8 | 5 |
| Completeness [%] | 98.0 | |
| Redundancy | 13.2 | 13.1 |
| CC(1/2) | 0.994 | 0.731 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.4 | 291 | 1.8 M Sodium phosphate monobasic monohydrate/Potassium phosphate dibasic pH 6.9 |
