9Z2H
Crystal structure of A10 Fab in complex with the EGFR peptide
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 19-ID |
| Synchrotron site | NSLS-II |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-03 |
| Detector | DECTRIS EIGER2 XE 9M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 129.617, 129.617, 144.898 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.250 - 2.450 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.22270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.789 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | BALBES |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.122 | |
| Rpim | 0.088 | 0.382 |
| Number of reflections | 46502 | 3566 |
| <I/σ(I)> | 4.286 | |
| Completeness [%] | 89.6 | |
| Redundancy | 2.4 | |
| CC(1/2) | 0.929 | 0.138 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.8M NaH2PO4, 1.2M KH2PO4, 0.1M Na Acetate, pH 4.5 |
