9Z1V
Backbone Modification in the GCN4 Leucine Zipper: Calpha-methyl-Lys at position 28
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER IMUS 3.0 MICROFOCUS |
| Temperature [K] | 150 |
| Detector technology | PIXEL |
| Collection date | 2025-06-12 |
| Detector | Bruker PHOTON III |
| Wavelength(s) | 1.541840 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.178, 30.434, 27.824 |
| Unit cell angles | 90.00, 96.93, 90.00 |
Refinement procedure
| Resolution | 27.620 - 1.650 |
| R-factor | 0.1959 |
| Rwork | 0.194 |
| R-free | 0.21130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.826 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.750 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmeas | 0.053 | 0.312 |
| Number of reflections | 7979 | 1256 |
| <I/σ(I)> | 29.4 | 5 |
| Completeness [%] | 99.7 | 98.9 |
| Redundancy | 8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 0.2 M sodium acetate pH 4.6, 0.1 M sodium citrate pH 4.6, 15% w/v PEG 3350 |
