9Z1Q
Backbone Modification in the GCN4 Leucine Zipper: Calpha-methyl-Glu at position 11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER IMUS 3.0 MICROFOCUS |
| Temperature [K] | 150 |
| Detector technology | PIXEL |
| Collection date | 2025-07-10 |
| Detector | Bruker PHOTON III |
| Wavelength(s) | 1.541840 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 79.156, 30.570, 27.396 |
| Unit cell angles | 90.00, 96.45, 90.00 |
Refinement procedure
| Resolution | 28.490 - 1.800 |
| R-factor | 0.1779 |
| Rwork | 0.176 |
| R-free | 0.19560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.604 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmeas | 0.059 | 0.298 |
| Number of reflections | 6143 | 965 |
| <I/σ(I)> | 18.8 | 4.3 |
| Completeness [%] | 99.5 | 98.4 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 0.3 M sodium acetate pH 4.6, 0.1 M sodium citrate pH 4.6, 5% w/v PEG 3350 |
