9Z1P
Backbone Modification in the GCN4 Leucine Zipper: Prototype
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER IMUS 3.0 MICROFOCUS |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-03-28 |
| Detector | Bruker PHOTON III |
| Wavelength(s) | 1.541840 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.178, 30.434, 27.824 |
| Unit cell angles | 90.00, 96.93, 90.00 |
Refinement procedure
| Resolution | 23.910 - 1.600 |
| R-factor | 0.1922 |
| Rwork | 0.188 |
| R-free | 0.22950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.933 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmeas | 0.053 | 0.442 |
| Number of reflections | 8725 | 1389 |
| <I/σ(I)> | 26.1 | 3.5 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 6.3 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M sodium acetate pH 4.5, 0.1 M sodium citrate pH 5.6, 10% w/v PEG 3350 |
