9Z0Z
Crystal Structure of the Poly(Hexamethylene Adipamide) (Nylon66) Hydrolase Nyl50 Acylenzyme Complex at Room Temperature
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2024-10-30 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 1.541838 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 53.810, 96.446, 104.663 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.390 - 3.100 |
| R-factor | 0.14676 |
| Rwork | 0.143 |
| R-free | 0.21843 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.819 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.390 | 3.310 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.265 | 0.587 |
| Rmeas | 0.280 | 0.619 |
| Rpim | 0.089 | 0.195 |
| Total number of observations | 98455 | 17990 |
| Number of reflections | 10389 | 1842 |
| <I/σ(I)> | 9.9 | 4.5 |
| Completeness [%] | 99.8 | |
| Redundancy | 9.5 | 9.8 |
| CC(1/2) | 0.983 | 0.903 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.4 | 291 | Imidazole 0.1 M pH 6.9, Polyethylene glycol 6,000 20.0% w/v |
