9Z0M
Human CD300c extracellular domain pH 7.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-11-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 30.329, 38.207, 82.366 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.180 - 1.700 |
| R-factor | 0.2036 |
| Rwork | 0.202 |
| R-free | 0.23930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.973 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (2.0_5761) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.180 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rpim | 0.026 | 0.669 |
| Number of reflections | 11124 | 574 |
| <I/σ(I)> | 14.7 | 1.5 |
| Completeness [%] | 100.0 | |
| Redundancy | 6.3 | |
| CC(1/2) | 0.999 | 0.459 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20% PEG1500, 0.1 M HEPES, pH 7.5 |
