9Z0F

Crystal structure of Trastuzumab Fc conjugated to Linker-Payload

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.180530
Spacegroup name	P 21 21 21
Unit cell lengths	49.458, 79.590, 138.112
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	68.960 - 2.460
R-factor	0.25
Rwork	0.249
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.513
Data reduction software	XDS (Feb 5, 2021 (BUILT 20210323))
Data scaling software	STARANISO (2.4.16)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.960	2.660
High resolution limit [Å]	2.460	2.460
Rmerge	0.119	2.118
Rmeas	0.122	2.217
Rpim	0.034	0.645
Number of reflections	15166	15162
<I/σ(I)>	15.8
Completeness [%]	93.4
Redundancy	12.9
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1 M MES, pH 6.5, 5% v/v MPD, 15% w/v PEG6000