9Z01

Crystal structure of a large RuBisCO from Promethearchaeum syntrophicum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00000
Spacegroup name	P 1
Unit cell lengths	69.550, 82.700, 113.300
Unit cell angles	108.17, 97.61, 103.24

Refinement procedure

Resolution	46.380 - 1.900
R-factor	0.1738
Rwork	0.173
R-free	0.20490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.720
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.380	1.930
High resolution limit [Å]	1.900	1.900
Number of reflections	161119	13462
<I/σ(I)>	6.3
Completeness [%]	99.5
Redundancy	3.8
CC(1/2)	0.994	0.311

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM Bis-Tris-HCl pH 6.5, 100 mM Ammonium phosphate dibasic, 20 % (w/v) PEG 3,350 and 5 % (v/v/) 2-Propanol.