9Z00

Kindlin-3/Integrin Beta3 Cytoplasmic Tail Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	90.250, 102.998, 111.845
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.146 - 2.199
R-factor	0.220099029122
Rwork	0.218
R-free	0.25362
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.535
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.146	2.278
High resolution limit [Å]	2.199	2.199
Rmerge	0.046
Number of reflections	53702	5300
<I/σ(I)>	22.13
Completeness [%]	100.0
Redundancy	5.5
CC(1/2)	0.986	0.889

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.2M sodium thiocyanate, 8% PEG3350, 30% ethylene glycol