9YYS

Crystal Structure of the Poly(Hexamethylene Adipamide) (Nylon66) Hydrolase Nyl10 at Cryo Temperature

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-07-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979460
Spacegroup name	C 1 2 1
Unit cell lengths	83.126, 120.193, 148.305
Unit cell angles	90.00, 92.99, 90.00

Refinement procedure

Resolution	68.400 - 1.300
R-factor	0.14387
Rwork	0.142
R-free	0.17013
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.694
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	74.050	74.050	1.320
High resolution limit [Å]	1.300	7.120	1.300
Rmerge	0.044	0.028	0.985
Rmeas	0.059	0.038	1.316
Rpim	0.039	0.025	0.865
Number of reflections	351281	2242	17449
<I/σ(I)>	10.7	33.3	1.4
Completeness [%]	98.8	99.8	99.2
Redundancy	3.9	4	3.8
CC(1/2)	0.999	0.998	0.440

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	4.7	293	0.5 M DL-malic acid pH 4.7, 15% w/v PEG 3350